< 1.0 EU per μg protein as determined by the LAL method.
Activity
Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 50-210 ng/mL.
Protein Construction
A DNA sequence encoding the human VEGF 183 isoform (P15692-3) (Met1-Arg209) was expressed.
Accession#
P15692-3
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Ala 27
Molecule Mass
The recombinant human VEGF 183 isoform consists of 183 amino acids and has a predicted molecular mass of 21 kDa.
Formulation
Lyophilized from sterile pH 7.92 100 mM glycine , 10 mM NaCl.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Related Pathways
VEGF Signaling Pathway
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.