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Project Description

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VEGF121 Protein, Human, Recombinant

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Specification

Cat. No.

NSC1215

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

VEGF121 Protein, Human, Recombinant

Size

10 µg

Price

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Synonyms

MVCD1 Protein, Human; VEGF Protein, Human; VEGF165 Protein, Human; VPF Protein, Human

Purity

> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

1. Immobilized human VEGF121 at 2μg/mL (100μL/well) can bind human VEGFR2-Fc, the EC50 is 6-35 ng/mL.2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).The ED50 for this effect is typically 3-15 ng/mL.

Protein Construction

A DNA sequence encoding the human VEGF 121 isoform (P15692-9) (Met1-Arg147) was expressed.

Accession#

P15692-9

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Ala 27

Molecule Mass

The recombinant human VEGF consists of 121 amino acids and has a predicted molecular mass of 14 kDa. The apparent molecular mass of the human VEGF 121 is approximately 19.9 and 17.0 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

VEGF Signaling Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.