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Project Description

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VEGF-C Protein, Human, Recombinant (His Tag)

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Specification

Cat. No.

NSC1253

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

VEGF-C Protein, Human, Recombinant (His Tag)

Size

5 µg

Price

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Synonyms

Flt4-L Protein, Human; LMPH1D Protein, Human; VEGF-C Protein, Human; VRP Protein, Human

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2 μg/mL (100 μL/well) can bind VEGFR3 hFc, the EC50 of VEGFR3 hFc is 2-15 ng/mL.

Protein Construction

A DNA sequence encoding the mature form of human VEGFC (NP_005420.1) (Thr103-Arg227) was expressed with a C-terminal polyhistidine tag.

Accession#

NP_005420.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Thr 103

Molecule Mass

The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.