< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1.Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is 0.2-0.8 ng/mL.2.The expression of IL11 and Acta2 mRNA in mouse fibroblasts was up-regulated after being cultured with 100ng/mL TGFB1 (Cat#80116-RNAH) for 24 hours. Data provided by Dr. Song at China Agricultural University.
Protein Construction
A DNA sequence encoding the mouse / rat TGFB1 (Ala279-Ser390) was expressed. Rat and Mouse mature TGFB1 sequences are identical.
Expressed Host
HEK293 Cells
Species
Mouse, Rat
Predicted N Terminal
Ala 279
Molecule Mass
The recombinant mouse / rat TGFB1 comprises 112 amino acids and predicts a molecular mass of 12.8 kDa.
Formulation
Lyophilized from sterile PBS, pH 3.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Related Pathways
P38 MAPK Signaling Pathway
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.