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Project Description

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TGF beta 1 Protein, Human, Recombinant (Latent, His Tag), Amine-Labeled Biotinylated

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Specification

Cat. No.

NSC1149

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

TGF beta 1 Protein, Human, Recombinant (Latent, His Tag), Amine-Labeled Biotinylated

Size

20 µg

Price

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Synonyms

CED Protein, Human; DPD1 Protein, Human; LAP Protein, Human; TGF-beta 1 Protein, Human; TGFB Protein, Human; TGFbeta Protein, Human

Purity

> 95 % as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the full length of human TGFβ1 (NP_000651.3) (Met1-Ser390) was fused with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

Accession#

NP_000651.3

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Leu 30 & Ala 279

Molecule Mass

The recombinant human latent TGFβ1 consists of 370 amino acids and predicts a molecular mass of 42.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TGFβ1 is approximately 16, 42 and 57 kDa corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

P38 MAPK Signaling Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.