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Project Description

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TGF-alpha Protein, Human, Recombinant

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Specification

Cat. No.

NSC977

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

TGF-alpha Protein, Human, Recombinant

Size

20 µg

Price

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Synonyms

TFGA Protein, Human; TGFa Protein, Human

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF-alpha at 2 μg/ml (100 μl/well) can bind Human EGFR hFc, the EC50 of Human EGFR hFc is 80-500 ng/mL. 2. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 ng/mL.

Protein Construction

A DNA sequence encoding the human TGFa (P01135-1) (Val40-Ala89) was expressed and purified.

Accession#

P01135-1

Expressed Host

E. coli

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.