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Project Description

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SPARCL1 Protein, Mouse, Recombinant (His Tag), HPLC-verified

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Specification

Cat. No.

NSC286

Category

Neural Stem Cell Differentiation Markers

Product Type

Proteins

Product Name

SPARCL1 Protein, Mouse, Recombinant (His Tag), HPLC-verified

Size

100 µg

Price

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Synonyms

Ecm2 Protein, Mouse; hevin Protein, Mouse; mast9 Protein, Mouse; Sc1 Protein, Mouse

Purity

≥ 85 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 μg/mL.

Protein Construction

A DNA sequence encoding the mouse SPARCL1 (EDL20231.1) (Met1-Phe650) was expressed with a C-terminal polyhistidine tag.

Accession#

EDL20231.1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Ile 17

Molecule Mass

The recombinant mouse SPARCL1 comprises 645 amino acids and has a predicted molecular mass of 72.2 kDa. The apparent molecular mass of the protein is approximately 118 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.