SPARCL1 Protein, Mouse, Recombinant (His Tag), HPLC-verified

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SPARCL1 Protein, Mouse, Recombinant (His Tag), HPLC-verified

Specification
Cat. No.
NSC286
Category
Neural Stem Cell Differentiation Markers
Product Type
Proteins
Product Name
SPARCL1 Protein, Mouse, Recombinant (His Tag), HPLC-verified
Size
100 µg
Price
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Synonyms
Ecm2 Protein, Mouse; hevin Protein, Mouse; mast9 Protein, Mouse; Sc1 Protein, Mouse
Purity
≥ 85 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 μg/mL.
Protein Construction
A DNA sequence encoding the mouse SPARCL1 (EDL20231.1) (Met1-Phe650) was expressed with a C-terminal polyhistidine tag.
Accession#
EDL20231.1
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Ile 17
Molecule Mass
The recombinant mouse SPARCL1 comprises 645 amino acids and has a predicted molecular mass of 72.2 kDa. The apparent molecular mass of the protein is approximately 118 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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