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Project Description

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Sonic Hedgehog/SHH Protein, Human, Recombinant (aa 1-197, His Tag)

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Specification

Cat. No.

NSC1116

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

Sonic Hedgehog/SHH Protein, Human, Recombinant (aa 1-197, His Tag)

Size

20 µg

Price

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Synonyms

HHG1 Protein, Human; HLP3 Protein, Human; HPE3 Protein, Human; MCOPCB5 Protein, Human; SMMCI Protein, Human; Sonic hedgehog Protein, Human; TPT Protein, Human; TPTPS Protein, Human

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells.

Protein Construction

A DNA sequence encoding the amino acid sequence (Met 1-Gly 197) of human SHH (Q15465), that is Sonic hedgehog protein N-product, was fused with a polyhistidine tag at the C-terminus.

Accession#

Q15465

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Cys 24

Molecule Mass

The recombinant human SHH (aa 1-197) consists of 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 24 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.