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Project Description

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S100B Protein, Human, Recombinant (hFc Tag), HPLC-verified

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Specification

Cat. No.

NSC506

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

S100B Protein, Human, Recombinant (hFc Tag), HPLC-verified

Size

100 µg

Price

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Synonyms

NEF Protein, Human; S100 Protein, Human; S100-B Protein, Human; S100beta Protein, Human

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

1. Measured by its ability to bind mouse S100A1 in a functional ELISA. 2. Measured by its ability to bind TP53 in a functional ELISA. 3. Immobilized recombinant human Fc-S100B at 10 μg/mL (100 μl/well) can bind biotinylated human S100A1 with a linear range of 15.6-250 ng/mL.

Protein Construction

A DNA sequence encoding the human S100B (NP_006263.1) (Ser 2-Glu 92) was expressed with the fused Fc region of human IgG1 at the N-terminus.

Accession#

NP_006263.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Glu 20

Molecule Mass

The recombinant human Fc/S100B is a disulfide-linked homodimeric protein. The reduced monomer consists of 328 amino acids and has a predicted molecular mass of 37.2 kDa. As a result of glycosylation, the apparent molecular mass of rhFc/S100B monomer is approximately 40 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.