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Project Description

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S100A1 Protein, Human, Recombinant (hFc Tag)

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Specification

Cat. No.

NRT016

Neurotransmitter Receptors, Transporters, and Ion Channels

S100A1

Product Type

Proteins

Product Name

S100A1 Protein, Human, Recombinant (hFc Tag)

Size

100 µg

Price

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Synonyms

S100 Protein, Human; S100-alpha Protein, Human; S100A Protein, Human

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to bind biotinylated Human Fc-S100B (cat: 10181-H01H) in functional Elisa.

Protein Construction

A DNA sequence encoding the human S100A1 (NP_006262.1) (Gly 2-Ser94) was expressed with the N-terminal fused Fc region of human IgG1.

Accession#

NP_006262.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Glu

Molecule Mass

The recombinant human Fc/S100A1 is a disulfide-linked homodimeric protein. The reduced monomer consists of 330 amino acids and has a predicted molecular mass of 37.1 kDa. As a result of glycosylation, the apparent molecular mass of rhFc/S100A1 monomer is approximately 40 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.