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Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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Recombinant Mouse Noggin/NOG Protein (hFc Tag)

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Specification

Cat. No.

NSC2536

Category

Neural Stem Cell Markers

Product Type

Proteins

Product Name

Recombinant Mouse Noggin/NOG Protein (hFc Tag)

Size

100 µg

Price

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Species

Mouse

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Molecule Mass

The recombinant mouse NOG/Fc comprises 454 amino acids and has a predicted molecular mass of 51 kDa. It migrates as approximately 60 kDa band in SDS-PAGE under reducing conditions.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Purity

> 85 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.1-0.6μg/mL

Protein Construction

A DNA sequence encoding the mouse NOG (P97466) (Met1-Cys232) was fused with the Fc region of human IgG1 at the C-terminus.

Accession#

P97466

Expressed Host

HEK293 Cells

Predicted N Terminal

Gln 28

Formulation

Lyophilized from sterile PBS, pH7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.