Inquiry

* Name

Phone

* Email

* Services or Products of Interest

Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

* Tick to confirm the Research Use Only (RUO) statement.

Recombinant Human Noggin/NOG Protein (hFc Tag)

Inquiry

Specification

Cat. No.

NSC2535

Category

Neural Stem Cell Markers

Product Type

Proteins

Product Name

Recombinant Human Noggin/NOG Protein (hFc Tag)

Size

100 µg

Price

Inquiry

Synonyms

Noggin Protein, Human; SYM1 Protein, Human; SYNS1 Protein, Human

Species

Human

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Molecule Mass

The recombinant human Noggin/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. Each monomer comprises 443 amino acids and has a predicted molecular mass of 49.8 kDa. As a result of glycosylation, the apparent molecular mass of rhNoggin/Fc monomer is approximately 58-62 kDa in SDS-PAGE under reducing conditions.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.1-0.6 μg/mL in the presence of 25 ng/mL of hBMP4.

Protein Construction

A DNA sequence encoding the human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was fused with the Fc region of human IgG1 at the C-terminus.

Accession#

NP_005441.1

Expressed Host

HEK293 Cells

Predicted N Terminal

Gln 28

Formulation

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.