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Project Description

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PSMA Protein, Human, Recombinant (ECD, His Tag)

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Specification

Cat. No.

NAE010

Neurotransmitter Associated Enzymes

PSMA

Product Type

Proteins

Product Name

PSMA Protein, Human, Recombinant (ECD, His Tag)

Size

50 µg

Price

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Synonyms

FGCP Protein, Human; FOLH Protein, Human; GCP2 Protein, Human; GCPII Protein, Human; mGCP Protein, Human; NAALAD1 Protein, Human; NAALAdase Protein, Human; PSM Protein, Human; PSMA Protein, Human

Purity

> 95 % as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >400 pmol/min/µg, as measured under the described conditions.

Protein Construction

A DNA sequence encoding the human FOLH1 (NP_004467.1) (Lys44-Ala750) was expressed with a polyhistidine tag at the N-terminus.

Accession#

NP_004467.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

His

Molecule Mass

The recombinant human FOLH1 consists 726 amino acids and predicts a molecular mass of 81.86 kDa. As a result of glycosylation, it migrates as an approximately 98.67 KDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 0.9 mM CaCl2, 0.5 mM MgCl2-6H2O, 2.7 mM KCl, 1.5 mM KH2PO4, 137.9 mM NaCl, 8.1 mM Na2HPO4-7H2O, 0.5 mM ZnCl2, pH 7.2.. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. . Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.