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Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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PLGF/PGF Protein, Mouse, Recombinant

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Specification

Cat. No.

NSC1204

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

PLGF/PGF Protein, Mouse, Recombinant

Size

50 µg

Price

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Synonyms

AI854365 Protein, Mouse; PIGF Protein, Mouse; Plgf Protein, Mouse

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its binding ability in a functional ELISA. Immobilized mouse PGF at 10 μg/mL (100ul/well) can bind human VEGFR1-His. The EC50 of human VEGFR1-His is 0.07-0.15 μg/mL.

Protein Construction

A DNA sequence encoding the mouse PGF (P49764) (Met1-Pro158) was fused with five amino acids (DDDDK) at the C-terminus was expressed and purified.

Accession#

P49764

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Val 19

Molecule Mass

The recombinant mouse PGF consists of 146 amino acids and has a calculated molecular mass of 16.6 kDa. The recombinant protein migrates as an approximately 28 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.