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Project Description

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PLGF/PGF Protein, Human, Recombinant (mFc Tag)

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Specification

Cat. No.

NSC1202

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

PLGF/PGF Protein, Human, Recombinant (mFc Tag)

Size

50 µg

Price

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Synonyms

D12S1900 Protein, Human; PGFL Protein, Human; PLGF Protein, Human; PlGF-2 Protein, Human; SHGC-10760 Protein, Human

Purity

> 95 % as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

1. Immobilized human PGF-mFc at 10 μg/mL (100 μL/well) can bind human VEGFR1-His. The EC50 of human VEGFR1-His is 0.19-0.43 μg/mL. 2. Labeled biotin to PLGF / PGF Protein, Human, Recombinant (Fc Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PLGF / PGF Protein, Human, Recombinant (Fc Tag), Biotinylated bound to Conbercept was 4.5 nM.

Protein Construction

A DNA sequence encoding the human PGF (NP_002623.2) (Met1-Arg170) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Accession#

NP_002623.2

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Leu 19

Molecule Mass

The recombinant human PGF consists 386 amino acids and predicts a molecular mass of 43.7 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.