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Project Description

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PDGF alpha receptor/PDGFRA Protein, Mouse, Recombinant (ECD, His Tag), HPLC-verified

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Specification

Cat. No.

NSC219

Category

Neural Stem Cell Differentiation Markers

Product Type

Proteins

Product Name

PDGF alpha receptor/PDGFRA Protein, Mouse, Recombinant (ECD, His Tag), HPLC-verified

Size

50 µg

Price

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Synonyms

AI115593 Protein, Mouse; CD140a Protein, Mouse; Pdgfr-2 Protein, Mouse

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse PDGFRA His at 2 μg/ml (100 μl/well) can bind Cynomolgus PDGF-B mFc, the EC50 of Cynomolgus PDGF-B mFc is 150-700 ng/mL.

Protein Construction

A DNA sequence encoding the Mouse PDGFRA (NP_001334647.1) (Met1-Ala528) was expressed with a polyhistide tag at the C-terminus.

Accession#

NP_001334647.1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Leu 25

Molecule Mass

The recombinant mouse PDGFRA consists of 515 amino acids and predicts a molecular mass of 57.87 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.