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Project Description

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PDGF alpha receptor/PDGFRA Protein, Human, Recombinant (hFc Tag)

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Specification

Cat. No.

NSC216

Category

Neural Stem Cell Differentiation Markers

Product Type

Proteins

Product Name

PDGF alpha receptor/PDGFRA Protein, Human, Recombinant (hFc Tag)

Size

100 µg

Price

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Synonyms

CD140A Protein, Human; PDGFR-2 Protein, Human; PDGFR2 Protein, Human; RHEPDGFRA Protein, Human

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to bind human PDGFC-His a functional ELISA.

Protein Construction

A DNA sequence encoding the extracellular domain (Met 1-Glu 524) of human PDGFRα (NP_006197.1) was fused with the Fc region of human IgG1 at the C-terminus.

Accession#

NP_006197.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Gln 24

Molecule Mass

The recombinant human PDGFRα/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 739 amino acids and predicts a molecular mass of 82.9 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhPDGFRα/Fc is approximately 120-130 kDa due to the glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.