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Project Description

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PDGF alpha receptor/PDGFRA Protein, Human, Recombinant (ECD, His & AVI Tag), Avi-tag Biotinylated

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Specification

Cat. No.

NSC217

Category

Neural Stem Cell Differentiation Markers

Product Type

Proteins

Product Name

PDGF alpha receptor/PDGFRA Protein, Human, Recombinant (ECD, His & AVI Tag), Avi-tag Biotinylated

Size

20 µg

Price

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Synonyms

CD140A Protein, Human; PDGFR-2 Protein, Human; PDGFR2 Protein, Human; RHEPDGFRA Protein, Human

Purity

> 95 % as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Immobilized Human PDGF-B Protein (His Tag) at 2 μg/mL (100 μL/well) can bind Human PDGFRA Protein (ECD, His & AVI Tag), Biotinylated, the EC50 is 400-1800 ng/mL.

Protein Construction

A DNA sequence encoding the human PDGFRA (NP_001334758.1) (Met1-Glu524) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Accession#

NP_001334758.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Gln 24

Molecule Mass

The recombinant human PDGFRA consists of 527 amino acids and predicts a molecular mass of 59.4 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Biotin/Protein Ratio

0.7-1 as determined by the HABA assay.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.