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Project Description

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NRG1 Beta 1 Protein, Human, Recombinant (EGF Domain, Fc Tag)

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Specification

Cat. No.

NSC950

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

NRG1 Beta 1 Protein, Human, Recombinant (EGF Domain, Fc Tag)

Size

100 µg

Price

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Synonyms

ARIA Protein, Human; GGF Protein, Human; GGF2 Protein, Human; HGL Protein, Human; HRG Protein, Human; HRG1 Protein, Human; HRGA Protein, Human; MST131 Protein, Human; MSTP131 Protein, Human; NDF Protein, Human; Neuregulin 1 Protein, Human; NRG1-IT2 Protein, Human; SMDF Protein, Human

Purity

> 86 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

1. Measured by its binding ability in a functional ELISA. 2. Immobilized Rhesus ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.58 μg/mL. 3. Immobilized human ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 μg/mL. 4.Human breast cancer organoids were cultured with FGF7, RSPO1, IGF1, EGF, NRG1 Beta 1, NOG. (Routinely tested). Data provided by D1 Medical Technology. 5.Human breast organoids were cultured with IGF1, NRG1 Beta 1. (Routinely tested). Data provided by D1 Medical Technology. 6.Human ovarian organoids were cultured with IGF1, NRG1 Beta 1, RSPO1, EGF, NOG. (Routinely tested). Data provided by D1 Medical Technology.

Protein Construction

A DNA sequence encoding the EGF-like domain (Thr 176-Lys 246) of human NRG1 isoform beta1 (Q02297-6) was fused with the Fc region of human IgG1 at the N-terminus.

Accession#

Q02297-6

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Glu

Molecule Mass

The recombinant human NRG1（aa176-246）/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 331 amino acids and has a calculated molecular mass of 36.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NRG1/Fc monomer is approximately 38 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.