NRG1 Beta 1 Protein, Human, Recombinant (ECD, Fc Tag)

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NRG1 Beta 1 Protein, Human, Recombinant (ECD, Fc Tag)

Specification
Cat. No.
NSC666
Category
Neural Stem Cell Growth Factors
Product Type
Proteins
Product Name
NRG1 Beta 1 Protein, Human, Recombinant (ECD, Fc Tag)
Size
100 µg
Price
Inquiry
Synonyms
ARIA Protein, Human; GGF Protein, Human; GGF2 Protein, Human; HGL Protein, Human; HRG Protein, Human; HRG1 Protein, Human; HRGA Protein, Human; MST131 Protein, Human; MSTP131 Protein, Human; NDF Protein, Human; Neuregulin 1 Protein, Human; NRG1-IT2 Protein, Human; SMDF Protein, Human
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Immobilized Human ERBB3 His at 2 μg/ml (100 μl/well) can bind Human NRG1 Fc, the EC50 of Human NRG1 Fc is 8-40 ng/mL. 2. Immobilized Rhesus ERBB3 His at 2 μg/ml (100 μl/well) can bind Human NRG1 Fc, the EC50 of Human NRG1 Fc is 20-80 ng/mL.
Protein Construction
A DNA sequence encoding the N-terminal fragment (Ser 2-Lys 246) of human NRG1 isoform beta1 (Q02297-6) was fused with the Fc region of human IgG1 at the N-terminus.
Accession#
Q02297-6
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu
Molecule Mass
The recombinant human NRG1(aa2-246)/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 505 amino acids and has a calculated molecular mass of 55.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 75 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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