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Project Description

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NGFR/p75NTR Protein, Rat, Recombinant (ECD, His Tag), HPLC-verified

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Specification

Cat. No.

NSC175

Category

Neural Stem Cell Differentiation Markers

Product Type

Proteins

Product Name

NGFR/p75NTR Protein, Rat, Recombinant (ECD, His Tag), HPLC-verified

Size

100 µg

Price

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Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the Rat NGFR (P07174) (Met1-Ile253) was expressed with a polyhistidine tag at the C-terminus.

Accession#

P07174

Expressed Host

HEK293 Cells

Species

Rat

Predicted N Terminal

Lys 30

Molecule Mass

The recombinant Rat NGFR consists of 235 amino acids and predicts a molecular mass of 25.34 kDa. It migrates as an approximately 59.0 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

Death Receptor Signaling

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.