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Project Description

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NGFR/p75NTR Protein, Mouse, Recombinant (hFc Tag)

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Specification

Cat. No.

NSC382

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

NGFR/p75NTR Protein, Mouse, Recombinant (hFc Tag)

Size

100 µg

Price

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Synonyms

LNGFR Protein, Mouse; p75 Protein, Mouse; p75NGFR Protein, Mouse; p75NTR Protein, Mouse; RP23-67E18.6 Protein, Mouse; Tnfrsf16 Protein, Mouse

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to inhibit NGF-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 2 ng/mL Recombinant mouse NGF.

Protein Construction

A DNA sequence encoding the extracellular domain of mouse NGFR (Q9Z0W1) (Met 1-Asn 253) was fused with the Fc region of human IgG1 at the C-terminus.

Accession#

Q9Z0W1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Lys 22

Molecule Mass

The secreted recombinant mouse NGFR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a calculated molecular mass of 50.6 kDa. The apparent molecular mass of rmNGFR/Fc monomer is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

Death Receptor Signaling

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.