Category
Neural Stem Cell Differentiation Markers
Product Name
NGFR/p75NTR Protein, Mouse, Recombinant (hFc Tag)
Synonyms
LNGFR Protein, Mouse; p75 Protein, Mouse; p75NGFR Protein, Mouse; p75NTR Protein, Mouse; RP23-67E18.6 Protein, Mouse; Tnfrsf16 Protein, Mouse
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to inhibit NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 2 ng/mL Recombinant mouse NGF.
Protein Construction
A DNA sequence encoding the extracellular domain of mouse NGFR (Q9Z0W1) (Met 1-Asn 253) was fused with the Fc region of human IgG1 at the C-terminus.
Expressed Host
HEK293 Cells
Predicted N Terminal
Lys 22
Molecule Mass
The secreted recombinant mouse NGFR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a calculated molecular mass of 50.6 kDa. The apparent molecular mass of rmNGFR/Fc monomer is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Related Pathways
Death Receptor Signaling