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Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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NCAM-1/CD56 Protein, Rat, Recombinant (His Tag), HPLC-verified

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Specification

Cat. No.

NSC476

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

NCAM-1/CD56 Protein, Rat, Recombinant (His Tag), HPLC-verified

Size

50 µg

Price

Inquiry

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the rat NCAM1 (P13596-1) (Met1-Thr721) was expressed with a polyhistidine tag at the C-terminus.

Accession#

P13596-1

Expressed Host

HEK293 Cells

Species

Rat

Predicted N Terminal

Leu 20

Molecule Mass

The recombinant rat NCAM1 comprises 713 amino acids and predicts a molecular mass of 79.2 kDa. The apparent molecular mass of the recombinant protein is approximately 114-118 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Formulation

Lyophilized from sterile 50 mM Tris, 0.15M NaCl, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.