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Project Description

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N-Cadherin Protein, Human, Recombinant (His Tag)

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Specification

Cat. No.

NSC1336

Category

Neural Stem Cell Markers

Product Type

Proteins

Product Name

N-Cadherin Protein, Human, Recombinant (His Tag)

Size

100 µg

Price

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Synonyms

CD325 Protein, Human; CDHN Protein, Human; CDw325 Protein, Human; NCAD Protein, Human

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 104 cells/well are added to CDH2 coated plates (10 μg/mL with 100 μL/well), approximately 60% will adhere after 1 hour at 37℃.

Protein Construction

A DNA sequence encoding the extracellular domain of human CDH2 (NP_001783.2) precursor (Met 1-Ala 724) was fused with a polyhistidine tag at the C-terminus.

Accession#

NP_001783.2

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Ser 26

Molecule Mass

The pro form of human CDH2 consists of 710 amino acids and predictes a molecular mass of 78.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of CDH2 is 90 & 75 kDa corresponding to the pro fom and mature form respectinely due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

Canonical Wnt Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.