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Project Description

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N-Cadherin Protein, Human, Recombinant (His & hFc Tag)

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Specification

Cat. No.

NSC1337

Category

Neural Stem Cell Markers

Product Type

Proteins

Product Name

N-Cadherin Protein, Human, Recombinant (His & hFc Tag)

Size

100 µg

Price

Inquiry

Synonyms

CD325 Protein, Human; CDHN Protein, Human; CDw325 Protein, Human; NCAD Protein, Human

Purity

> 70 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the human CDH2 (NP_001783.2) (Met 1-Ala 724) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Accession#

NP_001783.2

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Asp 160

Molecule Mass

The secreted recombinant human CDH2 is a disulfide-linked homodimeric protein. The reduced monomer comprises 813 amino acids and has a predicted molecular mass of 89.9 kDa. As a result of glycosylation, it migrates as an approximately 114 and 119 kDa band in SDS-PAGE under reducing conditions due to glycosylation.

Formulation

Lyophilized from sterile a 0.2 μm filtered solution in Tris and NaCl，pH 7.4，with Tween, Glycerol, and trehalose as protectants.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

Canonical Wnt Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.