Mouse SPARCL1 HEK293 Overexpression Lysate

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Mouse SPARCL1 HEK293 Overexpression Lysate

Specification
Cat. No.
NSC297
Category
Neural Stem Cell Differentiation Markers
Product Type
Lysates
Product Name
Mouse SPARCL1 HEK293 Overexpression Lysate
Size
300 µg
Price
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Synonyms
Mouse Ecm2 Overexpression Lysate; Mouse hevin Overexpression Lysate; Mouse mast9 Overexpression Lysate; Mouse Sc1 Overexpression Lysate
Species
Mouse
Molecule Mass
The recombinant mouse Sparcl1 consists 302 amino acids and predicts a molecular mass of 34.8 kDa.
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Product Description
This Mouse SPARCL1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SPARCL1 protein (Cat: 50544-M07H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Sequence Information
A DNA sequence encoding the mouse Sparcl1 (NP_034227.3) (Tyr368-Phe650) was expressed with a polyhistidine tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Application
Western Blot (WB)Optimal dilutions/concentrations should be determined by the end user.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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