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Mouse SFRP2 HEK293 Overexpression Lysate

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Specification

Cat. No.

NSC2546

Category

Neural Stem Cell Markers

Product Type

Lysates

Product Name

Mouse SFRP2 HEK293 Overexpression Lysate

Size

300 µg

Price

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Synonyms

Mouse AI851596 Overexpression Lysate; Mouse Sdf5 Overexpression Lysate

Species

Mouse

Application

Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.

Product Description

This Mouse SFRP2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SFRP2 protein from the overexpression lysate was verified.

Expression Host

HEK293 Cells

Sequence Information

A DNA sequence encoding the mouse sFRP2 (NP_033170.1) (Met 1-Cys 295) was expressed with a C-terminal polyhistidine tag.

Molecule Mass

The recombinant mouse sFRP2 consists of 282 amino acids after removal of the signal peptide and has a predicted molecular mass of 32.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 36 kDa due to glycosylation.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Lysis Buffer

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.

Sample Buffer

1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

Stability & Storage

Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.