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Mouse N-Cadherin HEK293 Overexpression Lysate

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Specification

Cat. No.

NSC1352

Category

Neural Stem Cell Markers

Product Type

Lysates

Product Name

Mouse N-Cadherin HEK293 Overexpression Lysate

Size

300 µg

Price

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Synonyms

Mouse CDHN Overexpression Lysate; Mouse N-cadherin Overexpression Lysate; Mouse Ncad Overexpression Lysate

Species

Mouse

Molecule Mass

The recombinant mouse CDH2 consists of 706 amino acids and has a calculated molecular mass of 77.8 kDa. The recombinant protein migrates as an approximately 91 and 114 kDa band in SDS-PAGE under reducing conditions due to glycosylation.

Stability & Storage

Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.

Product Description

This Mouse N-Cadherin overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of N-Cadherin protein (Cat: 50752-MCCH) from the overexpression lysate was verified.

Expression Host

HEK293 Cells

Sequence Information

A DNA sequence encoding the mouse CDH2 (P15116) (Met1-Ala724) was fused with six amino acids (LEVLFQ) at the C-terminus was expressed and purified.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Lysis Buffer

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.

Sample Buffer

1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

Application

Western Blot (WB)Optimal dilutions/concentrations should be determined by the end user.

Related Pathways

Canonical Wnt Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.