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Mouse Butyrylcholinesterase HEK293 Overexpression Lysate

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Specification

Cat. No.

NAE447

Neurotransmitter Associated Enzymes

Butyrylcholinesterase

Product Type

Lysates

Product Name

Mouse Butyrylcholinesterase HEK293 Overexpression Lysate

Size

300 µg

Price

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Product Description

This Mouse Butyrylcholinesterase overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Butyrylcholinesterase protein from the overexpression lysate was verified.

Expression Host

HEK293 Cells

Species

Mouse

Sequence Information

A DNA sequence encoding the mouse BCHE (NP_033868.3) (Met 1-Leu 603) was expressed, with a C-terminal polyhistidine tag.

Molecule Mass

The secreted recombinant mouse BCHE consists of 587 amino acids and has a calculated molecular mass of 66.7 kDa. The apparent molecular mass of the recombinant protein is approximately 60-70 kDa in SDS-PAGE under reducing conditions.

Stability & Storage

Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Lysis Buffer

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.

Sample Buffer

1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

Synonyms

Mouse C730038G20Rik Overexpression Lysate

Application

Western Blot (WB). Optimal dilutions/concentrations should be determined by the end user.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.