Mouse Betacellulin HEK293 Overexpression Lysate

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Mouse Betacellulin HEK293 Overexpression Lysate

Specification
Cat. No.
NSC875
Category
Neural Stem Cell Growth Factors
Product Type
Lysates
Product Name
Mouse Betacellulin HEK293 Overexpression Lysate
Size
300 µg
Price
Inquiry
Synonyms
Mouse Bcn Overexpression Lysate
Species
Mouse
Molecule Mass
The recombinant mouse BTC/Fc is a disulfide-linked homodimer. The reduced monomer consists of 335 amino acids and has a predicted molecular mass of 38 kDa. As a result of glycosylation, the apparent molecular mass of rm BTC/Fc monomer migrates with an apparent molecular mass of 50-55 kDa in SDS-PAGE under reducing conditions.
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Product Description
This Mouse Betacellulin overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Betacellulin protein (Cat: 50340-M03H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Sequence Information
A DNA sequence encoding the mouse BTC (NP_031594.1) extracellular domain (Met 1-Gln 118) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Application
Western Blot (WB)Optimal dilutions/concentrations should be determined by the end user.
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