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Project Description

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Leptin Protein, Mouse, Recombinant (mFc Tag)

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Specification

Cat. No.

NAE025

Neurotransmitter Associated Enzymes

Leptin

Product Type

Proteins

Product Name

Leptin Protein, Mouse, Recombinant (mFc Tag)

Size

50 µg

Price

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Synonyms

ob Protein, Mouse; obese Protein, Mouse

Purity

> 85 % as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the mouse Leptin (P41160) (Met1-Cys167) was expressed with the Fc region of mouse IgG2a at the C-terminus.

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Val 22

Molecule Mass

The recombinant mouse Leptin consists of 394 amino acids and predicts a molecular mass of 43.33 kDa. It migrates as an approximately 50.13 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. . Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

AMPK Signaling Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.