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Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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Leptin Protein, Human, Recombinant

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Specification

Cat. No.

NAE024

Neurotransmitter Associated Enzymes

Leptin

Product Type

Proteins

Product Name

Leptin Protein, Human, Recombinant

Size

100 µg

Price

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Synonyms

LEPD Protein, Human; OB Protein, Human; OBS Protein, Human

Purity

> 98 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

Immobilized Human Leptin(Cat:10221-HNAE) at 2 μg/mL (100 μL/well) can bind Human Leptin Receptor (His & hFc Tag)(Cat:10322-H03H), the EC50 of Human Leptin Receptor (His & hFc Tag)(Cat:10322-H03H) is 70-420 ng/mL.

Protein Construction

A DNA sequence encoding the mature form of human Leptin (NP_000221.1) (Val 22-Cys 167) was expressed, with an additional Met.

Accession#

NP_000221.1

Expressed Host

E. coli

Species

Human

Predicted N Terminal

Met

Molecule Mass

The recombinant human leptin consists of 147 amino acids and predicts a molecular mass of 16 kDa. The apparent molecular mass of rhLEP is approximately 13 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. . Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

AMPK Signaling Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.