Measured by its ability to inhibit carboxypeptidase-A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis(2-nitrobenzoic acid) (DTNB). The IC50 value is <2.0 nM .
Protein Construction
A DNA sequence encoding the mouse LXN (NP_058033.2) (Glu 2-Glu 222) was expressed, with a polyhistide tag at the N-terminus.
Accession#
NP_058033.2
Expressed Host
E. coli
Species
Mouse
Predicted N Terminal
Met
Molecule Mass
The recombinant mouse LXN consisting of 228 amino acids and has a calculated molecular mass of 26.3 kDa. The apparent molecular mass of the rmLXN is approximately 32 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 8.0, 10% glycerolPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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