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Project Description

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L1CAM Protein, Human, Recombinant (His Tag), HPLC-verified

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Specification

Cat. No.

NSC597

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

L1CAM Protein, Human, Recombinant (His Tag), HPLC-verified

Size

100 µg

Price

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Synonyms

CAML1 Protein, Human; CD171 Protein, Human; HSAS Protein, Human; HSAS1 Protein, Human; MASA Protein, Human; MIC5 Protein, Human; N-CAM-L1 Protein, Human; N-CAML1 Protein, Human; NCAM-L1 Protein, Human; S10 Protein, Human; SPG1 Protein, Human

Purity

≥ 85 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the extracellular domain of human CD171 (NP_000416.1) (Met 1-Glu 1120) was fused with a polyhistidine tag at the C-terminus.

Accession#

NP_000416.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Ile 20

Molecule Mass

The recombinant human CD171 consists of 1112 amino acids after removal of the signal peptide and predicts a molecular mass of 125 kDa. As a result of glycosylation, the apparent molecular mass of rhCD171 is approximately 160-200 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.