L1CAM Protein, Human, Recombinant (His & AVI Tag), Avi-tag Biotinylated, HPLC-verified

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L1CAM Protein, Human, Recombinant (His & AVI Tag), Avi-tag Biotinylated, HPLC-verified

Specification
Cat. No.
NSC596
Category
Neural Stem Cell Growth Factors
Product Type
Proteins
Product Name
L1CAM Protein, Human, Recombinant (His & AVI Tag), Avi-tag Biotinylated, HPLC-verified
Size
20 µg
Price
Inquiry
Synonyms
CAML1 Protein, Human; CD171 Protein, Human; HSAS Protein, Human; HSAS1 Protein, Human; MASA Protein, Human; MIC5 Protein, Human; N-CAM-L1 Protein, Human; N-CAML1 Protein, Human; NCAM-L1 Protein, Human; S10 Protein, Human; SPG1 Protein, Human
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the Human L1CAM (NP_000416.1) (Met1-Glu1120) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Accession#
NP_000416.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ile 20
Molecule Mass
The recombinant Human L1CAM consists of 1127 amino acids and predicts a molecular mass of 126.46 kDa. It migrates as an approximately 145.78 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Biotin/Protein Ratio
0.7-1 as determined by the HABA assay.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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