Inquiry

* Name

Phone

* Email

* Services or Products of Interest

Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

* Tick to confirm the Research Use Only (RUO) statement.

L1CAM Protein, Human, Recombinant (His & AVI Tag), Avi-tag Biotinylated, HPLC-verified

Inquiry

Specification

Cat. No.

NSC596

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

L1CAM Protein, Human, Recombinant (His & AVI Tag), Avi-tag Biotinylated, HPLC-verified

Size

20 µg

Price

Inquiry

Synonyms

CAML1 Protein, Human; CD171 Protein, Human; HSAS Protein, Human; HSAS1 Protein, Human; MASA Protein, Human; MIC5 Protein, Human; N-CAM-L1 Protein, Human; N-CAML1 Protein, Human; NCAM-L1 Protein, Human; S10 Protein, Human; SPG1 Protein, Human

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the Human L1CAM (NP_000416.1) (Met1-Glu1120) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Accession#

NP_000416.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Ile 20

Molecule Mass

The recombinant Human L1CAM consists of 1127 amino acids and predicts a molecular mass of 126.46 kDa. It migrates as an approximately 145.78 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Biotin/Protein Ratio

0.7-1 as determined by the HABA assay.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.