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Project Description

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Kininogen 1 Protein, Human, Recombinant (His Tag), HPLC-verified

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Specification

Cat. No.

NAE022

Neurotransmitter Associated Enzymes

Kininogen 1

Product Type

Proteins

Product Name

Kininogen 1 Protein, Human, Recombinant (His Tag), HPLC-verified

Size

50 µg

Price

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Synonyms

BDK Protein, Human; BK Protein, Human; KNG Protein, Human

Purity

≥ 85 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC, R&D Systems, Catalog # ES009 . The IC50 value is < 7 nM .

Protein Construction

A DNA sequence encoding the human KNG1 isoform 1 (NP_001095886.1) (Gln 19-Ser 644) was fused with a polyhistidine tag at the C-terminus, and a signal peptide at the N-terminus.

Accession#

NP_001095886.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Gln 19

Molecule Mass

The recombinant human KNG1 consists of 637 amino acids and has a predicted molecular mass of 71.3 kDa. As a result of glycosylation, the apparent molecular mass of rhKNG1 is approximately 100-110 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 25mM Tris, 100mM NaCl, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. . Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.