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Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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KGF/FGF-7 Protein, Human, Recombinant (His Tag), HPLC-verified

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Specification

Cat. No.

NSC1078

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

KGF/FGF-7 Protein, Human, Recombinant (His Tag), HPLC-verified

Size

20 µg

Price

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Synonyms

HBGF-7 Protein, Human; KGF Protein, Human

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Endotoxin

< 0.1 EU per μg of the protein.

Activity

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The ED50 for this effect is typically 3-20 ng/mL.

Protein Construction

A DNA sequence encoding the human KGF (P21781) (Cys32-Thr194) was expressed with a polyhistidine tag at the N-terminus.

Accession#

P21781

Expressed Host

E. coli

Species

Human

Predicted N Terminal

His

Molecule Mass

The recombinant human KGF consists of 179 amino acids and predicts a molecular mass of 21 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 50mM Tris，250mM NaCl，pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.