Ace Therapeutics offers custom in situ Hybridization (ISH) services, including probe design, tissue procurement and professionally interpreted gene expression studies. Our scientists are highly trained in ISH and can bring you highly sensitive nucleic acid detection in your samples to accelerate your research in psychiatry.

Introduction of In Situ Hybridization in Psychiatry

In situ hybridization has played an important role in uncovering the neuroanatomical distribution and characterizing many physiologically important neuropeptides such as NPY, Orexin, CRF and NPS. These neuropeptides have been found to be downstream factors in the progression of many psychiatric disorders. Unlike other genomic expression techniques such as RT-PCR, in situ hybridization has advantages in that it not only measures the expression levels of genes, but also provides a wealth of information about their spatial location and expression patterns. These gene patterns can be associated with specific cellular phenotypes, which is important in the study of psychiatric disease progression.

Fig. 1 In situ hybridization of brain tissue. (Matzke A, et al., 2007)

In Situ Hybridization Services

Ace Therapeutics focuses on two basic methods to visualize in situ RNA and DNA targets, namely fluorescent (FISH) and colorimetric (CISH) assays. The characteristics of each assay itself (see table below) make FISH and CISH suitable for very different applications. We will help you choose the right method for your research, depending on the purpose of your experiment.

In Situ Hybridization Probes

• Double-stranded DNA (dsDNA) probes
• Single-stranded DNA (ssDNA) probes
• RNA probes (riboprobes)
• Synthetic oligonucleotides (PNA, LNA)

What Can We Help You Achieve in Psychiatry with ISH?

• Molecular cytogenetic analysis of psychiatric disorders such as schizophrenia
• Study of chromosome aneuploidy, abnormal patterns of chromosome organization and expression of functional genes in situ in brain neurons of different psychiatric and neurodevelopmental disorders
• Study of the pathogenesis of psychiatric disorders such as schizophrenia
• Detection of quantitative changes in gene expression in different neuroanatomical regions

Our Advantages

• High detection sensitivity and short time required to analyze results
• Global reach with multi-site support
• Provides advice on biomarker analysis strategy and implementation
• Provides a secure web portal that allows you to view all images and analyses in real time

Service Process

Ace Therapeutics' bioinformatics experts are excited to work with you to design probes for your application. We work with you to select the ideal probe length, location and labeling chemistry to ensure the best in situ hybridization results. If you are interested in ICH, please feel free to make an inquiry.

Reference

1. Matzke A, et al. Haploinsufficiency of c-Met in cd44^-/- mice identifies a collaboration of CD44 and c-Met in vivo. Mol Cell Biol. 2007, 27(24):8797-8806.