≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Endotoxin
Please contact us for more information.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human IGF1 at 2 μg/ml (100 μl/well) can bind Human IGFBP4 His, the EC50 of Human IGFBP4 His is 10-60 ng/mL.
Protein Construction
A DNA sequence encoding the human IGF1 (P05019-1) (Gly49-Ala118) was expressed.
Accession#
P05019-1
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Gly 49
Molecule Mass
The recombinant human IGF1 consists of 71 amino acids and has a calculated molecular mass of 7.8 KDa.
Formulation
Lyophilized from sterile 30% Acetonitrile, 0.1% TFAPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.