Human PDGF alpha receptor/PDGFRA HEK293 Overexpression Lysate

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Human PDGF alpha receptor/PDGFRA HEK293 Overexpression Lysate

Specification
Cat. No.
NSC235
Category
Neural Stem Cell Differentiation Markers
Product Type
Lysates
Product Name
Human PDGF alpha receptor/PDGFRA HEK293 Overexpression Lysate
Size
300 µg
Price
Inquiry
Synonyms
Human CD140A Overexpression Lysate; Human PDGFR-2 Overexpression Lysate; Human PDGFR2 Overexpression Lysate; Human RHEPDGFRA Overexpression Lysate
Species
Human
Molecule Mass
The recombinant human PDGFRα consists of 507 amino acids and predicts a molecular mass of 56.8 kDa. By SDS-PAGE, the apparent molecular mass of rhPDGFRα is approximately 90-100 kDa due to the glycosylation.
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Product Description
This Human PDGF alpha receptor/PDGFRA overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PDGF alpha receptor/PDGFRA protein (Cat: 10556-HCCH) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Sequence Information
The mature form of human PDGFRa (NP_006197.1) extracellular domain (Met 1-Glu 524) with five amino acids (DDDDK) at the C-terminus was expressed and purified.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Application
Western Blot (WB)Optimal dilutions/concentrations should be determined by the end user.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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