Neurotransmitter Receptors, Transporters, and Ion Channels
DPP10
Product Name
Human DPP10 HEK293 Overexpression Lysate
Synonyms
Human DPL2 Overexpression Lysate; Human DPPY Overexpression Lysate; Human DPRP-3 Overexpression Lysate; Human DPRP3 Overexpression Lysate
Description
This Human DPP10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control.
Molecule Mass
The recombinant human DPP10 consists of 760 amino acids and has a calculated molecular mass of 87.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhDPP10 is approximately 90-110 kDa due to glycosylation.
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB). Optimal dilutions/concentrations should be determined by the end user.
Expression Host
HEK293 Cells
Sequence Information
A DNA sequence encoding the human DPP10 isoform 1 (Q8N608-1) extracellular domain (Leu 56-Glu 796) was expressed, with a polyhistidine tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).