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Human CD44 HEK293 Overexpression Lysate

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Specification

Cat. No.

NRT1014

Neurotransmitter Receptors, Transporters, and Ion Channels

CD44

Product Type

Lysates

Product Name

Human CD44 HEK293 Overexpression Lysate

Size

300 µg

Price

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Synonyms

Human CDW44 Overexpression Lysate; Human CSPG8 Overexpression Lysate; Human ECMR-III Overexpression Lysate; Human HCELL Overexpression Lysate; Human HUTCH-I Overexpression Lysate; Human IN Overexpression Lysate; Human LHR Overexpression Lysate; Human MC56 Overexpression Lysate; Human MDU2 Overexpression Lysate; Human MDU3 Overexpression Lysate; Human MIC4 Overexpression Lysate; Human Pgp1 Overexpression Lysate

Description

This Human CD44 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control.

Species

Human

Molecule Mass

The recombinant human CD44 consists of 211 amino acids and has a predicted molecular mass of 23.4 kDa. As a result of glycosylation, the apparent molecular mass of rhCD44 is approximately 43-48 kDa in SDS-PAGE under reducing conditions.

Stability & Storage

Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.

Application

Western Blot (WB). Optimal dilutions/concentrations should be determined by the end user.

Expression Host

HEK293 Cells

Sequence Information

A DNA sequence encoding the N-terminal fragment (Met 1-Pro 220) of human CD44 (P16070-1) extracellular domain was fused with a polyhistidine tag at the C-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Lysis Buffer

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.

Sample Buffer

1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.