Human ATF2 Baculovirus-Insect cells Overexpression Lysate
Size
300 µg
Price
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Synonyms
Human CRE-BP1 Overexpression Lysate; Human CREB-2 Overexpression Lysate; Human CREB2 Overexpression Lysate; Human HB16 Overexpression Lysate; Human TREB7 Overexpression Lysate
Species
Human
Application
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Product Description
This Human ATF2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of ATF2 protein from the overexpression lysate was verified.
Expression Host
Baculovirus-Insect cells
Sequence Information
A DNA sequence encoding full length of human ATF2 (P15336-1) (Met 1-Ser 505) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Molecule Mass
The recombinant human ATF2/GST chimera consists of 742 amino acids and has a calculated molecular mass of 82.4 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
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