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Project Description

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HB-EGF Protein, Human, Recombinant

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Specification

Cat. No.

NSC934

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

HB-EGF Protein, Human, Recombinant

Size

10 µg

Price

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Synonyms

DTR Protein, Human; DTS Protein, Human; DTSF Protein, Human; HEGFL Protein, Human

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts.The ED50 for this effect is typically 0.4-2ng/mL.

Protein Construction

A DNA sequence encoding the human HBEGF (Q99075) (Met 1-Leu 148) without the C-terminal propeptide (aa 149-208), was expressed and purified.

Accession#

Q99075

Expressed Host

Baculovirus-Insect Cells

Species

Human

Predicted N Terminal

Asp 63

Molecule Mass

The expressed pro form of human HBEGF consists of 148 amino acids and predicts a molecular mass of 16.4 kDa. The processed soluble HBEGF is a 9.7 kDa protein comprising 86 amino acids generated by proteolytic processing.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.