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Project Description

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GDNF Protein, Human, Recombinant

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Specification

Cat. No.

NSC1099

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

GDNF Protein, Human, Recombinant

Size

10 µg

Price

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Synonyms

ATF1 Protein, Human; ATF2 Protein, Human; HFB1-GDNF Protein, Human; HSCR3 Protein, Human

Purity

> 97 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-20 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 His Chimera.

Protein Construction

A DNA sequence encoding the mature form of human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.

Accession#

P39905-2

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Gly

Molecule Mass

The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.