Inquiry

* Name

Phone

* Email

* Services or Products of Interest

Project Description

* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

* Tick to confirm the Research Use Only (RUO) statement.

GAD65 Protein, Mouse, Recombinant (His & GST Tag)

Inquiry

Specification

Cat. No.

NAE017

Neurotransmitter Associated Enzymes

GAD65

Product Type

Proteins

Product Name

GAD65 Protein, Mouse, Recombinant (His & GST Tag)

Size

50 µg

Price

Inquiry

Synonyms

6330404F12Rik Protein, Mouse; GAD(65) Protein, Mouse; Gad-2 Protein, Mouse; GAD65 Protein, Mouse

Purity

> 92 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Testing in progress

Protein Construction

A DNA sequence encoding full length of mouse GAD2 (NP_032104.2) (Met 1-Leu 585) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Accession#

NP_032104.2

Expressed Host

Baculovirus-Insect Cells

Species

Mouse

Predicted N Terminal

Met

Molecule Mass

The recombinant mouse GAD2/GST chimera consists of 822 amino acids and has a calculated molecular mass of 93 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM GSH, 3mM DTT, 10% gly, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. . Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.