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Project Description

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GABARAP Protein, Human, Recombinant (His & MBP Tag)

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Specification

Cat. No.

NRT051

Neurotransmitter Receptors, Transporters, and Ion Channels

GABARAP

Product Type

Proteins

Product Name

GABARAP Protein, Human, Recombinant (His & MBP Tag)

Size

100 µg

Price

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Synonyms

ATG8A Protein, Human; GABARAP-a Protein, Human; MM46 Protein, Human

Purity

> 85 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the human GABARAP (NP_009209.1) (Met1-Leu117) was expressed with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

Accession#

NP_009209.1

Expressed Host

E. coli

Species

Human

Predicted N Terminal

His

Molecule Mass

The recombinant human GABARAP consists of 514 amino acids and predicts a molecular mass of 57.5 KDa. It migrates as an approximately 49-58 KDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.