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Project Description

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FGF1 Protein, Mouse, Rat, Recombinant

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Specification

Cat. No.

NSC998

Category

Neural Stem Cell Growth Factors

Product Type

Proteins

Product Name

FGF1 Protein, Mouse, Rat, Recombinant

Size

10 µg

Price

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Purity

> 90 % as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Activity

Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.04-0.2 ng/mL.

Protein Construction

A DNA sequence encoding the mature form of mouse / rat FGF1 (NP_034327.1) (Phe16-Asp155) was expressed. Mouse and Rat FGF1 sequences are identical.

Accession#

NP_034327.1

Expressed Host

E. coli

Species

Mouse, Rat

Predicted N Terminal

Met

Molecule Mass

The recombinant mouse / rat FGF1 consisting of 141 amino acids and has a calculated molecular mass of 16 kDa as estimated in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 50mM Na2SO4, 0.5mM DTT, 1mM EDTA, 20mM Tris, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Related Pathways

Actin Dynamics Signaling Pathway G Protein-coupled Receptors Signaling MAPK-Erk Pathway Autophagy Pathway

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.