Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts.The ED50 for this effect is typically 0.1-0.4 ng/mL.
Protein Construction
A DNA sequence encoding the cyno FGF1 (Phe16-Asp155) was expressed and purified with an initial Met.
Expressed Host
E. coli
Species
Cynomolgus
Predicted N Terminal
Met
Molecule Mass
The recombinant cyno FGF1 consists of 141 amino acids and has a calculated molecular mass of 16 kDa. It migrates as an 16 KDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile PBS, pH 7.4, 20% glycerol.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping
Liquid. It is shipped out with blue ice.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.